Morphologic and histochemical features of human sarcomas in tissue culture.

The portions of neoplasm used for expiants were cut into 1to 2-mm. fragments. The clot was formed by mixing 3 drops of chicken blood plasma, one drop of embryo extract, and 1 per cent throm bin. Three expiants were placed on separate slides. Sixteen to 24 double slides (back to back) were incubated in roller tubes with either human ascitic or serum nutrient fluid. 1. Ascitic fluid medium, 50 per cent Gey's BSS